FastQCFastQC Report
Sat 31 Dec 2022
SRR1954730.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954730.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358860
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.8384885470657081No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT28360.7902803321629605Illumina PCR Primer Index 10 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC23250.6478849690687176No Hit
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC21700.6046926377974697Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG21430.5971688123502201No Hit
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC18270.5091121885972246Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC14720.41018781697597945No Hit
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC11860.3304909992754835Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC9100.2535807835924873No Hit
ACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC8440.23518921027698825Illumina PCR Primer Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT6940.19339018001449032No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC6130.17081870367274146No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.15437775176949228No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTTCTCT5410.15075516914674245No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT5240.1460179457169927Illumina PCR Primer Index 10 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5130.14295268349774284No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG5110.1423953630942429Illumina PCR Primer Index 10 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTA4130.11508666332274424No Hit
GAATATGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC3900.10867747868249457No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC3870.10784149807724461No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG3780.10533355626149474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)