FastQCFastQC Report
Sat 31 Dec 2022
SRR1954729.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954729.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139636
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.5736343063393394No Hit
CTGTCTATTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT5880.42109484660116303Illumina PCR Primer Index 10 (95% over 24bp)
GAATCTATCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC5210.3731129508149761No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG4520.32369875963218653No Hit
CCTGTCACTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC4290.30722736257125666Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCACTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC3860.2764330115443009Illumina PCR Primer Index 10 (95% over 23bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC3480.24921939900885157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.16829470910080496No Hit
TCTGTCACTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC2220.15898478902288807Illumina PCR Primer Index 10 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.14967486894497123No Hit
GAATGAATGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT1530.10957059784009854No Hit
GAATGAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC1490.10670600704689336No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC1450.10384141625368816No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG1420.10169297315878426No Hit
ACTGTCACTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC1410.10097682546048298Illumina PCR Primer Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)