FastQCFastQC Report
Sat 31 Dec 2022
SRR1954728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275031
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48681.7699822929051634No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG9940.361413804262065No Hit
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT9870.35886863662641666TruSeq Adapter, Index 16 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC9640.35050594296642923No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC9520.3461427984481749TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9280.33741650941166634No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC8680.3156007868203948No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC8410.3057837116543226TruSeq Adapter, Index 13 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.3043293301482378No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC6870.249790023670059No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC3160.11489613898069673No Hit
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC3070.11162378059200598TruSeq Adapter, Index 13 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2950.10726063607375169No Hit
ACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC2850.10362468230853976TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)