FastQCFastQC Report
Sat 31 Dec 2022
SRR1954727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113606
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12351.0870904705737374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4100.3608964315265039No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.33008819956692426No Hit
GAATCTATCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC2500.22005879971128284No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG2310.20333433093322537No Hit
CTGTCTATTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT2050.18044821576325196TruSeq Adapter, Index 16 (95% over 22bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC2040.17956798056440681No Hit
GCTGTCACTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC1970.17340633417249088TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCACTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC1830.16108304138865906TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.11619104624755734No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1190.10474798866257065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)