FastQCFastQC Report
Sat 31 Dec 2022
SRR1954726.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954726.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322855
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34141.0574406467299562No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT21460.6646946771770609Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC17630.5460655712316674Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC16640.515401650895913No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10700.3314181288813864No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC10510.32553313406947393Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC7490.23199269021697047No Hit
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC7280.22548822226696194Illumina Single End Adapter 2 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.18181536603119047No Hit
ACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC5360.16601880100974123Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG4500.1393814560716111No Hit
AAAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC3250.10066438494060802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)