FastQCFastQC Report
Sat 31 Dec 2022
SRR1954725.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954725.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134781
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.6981696233148589No Hit
CTGTCTATTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT5110.3791335574005238Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.3628107819351392No Hit
CCTGTCACTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC3720.2760032942328666Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC2560.18993775086992973Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.18771191785192273No Hit
CGTTTTATTTCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCC1970.14616303484912563No Hit
TCTGTCACTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC1690.12538859334772703Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)