FastQCFastQC Report
Sat 31 Dec 2022
SRR1954721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134856
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.6510648395325385No Hit
GAATCTATCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC7730.5732040102034763No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG6400.47458029305333094No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC4500.33368926855312336No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.1972474343002907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.1876075220976449No Hit
GCTGTCACTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC2520.18686599038974908No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC2210.16387850744497834No Hit
CTGTCTATTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT2160.16017084890549918No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG1990.1475648098712701No Hit
GAATGAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC1920.1423740879159993No Hit
GAATGAATGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT1860.1379248976686243No Hit
GAACTGACTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT1670.12383579521860355No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1640.12161120009491605No Hit
GGACATATTTATAAACATCTTAATCTCAACACATAATTTTTGGAATAAAAC1640.12161120009491605No Hit
CCTGTCACTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC1600.11864507326333273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)