FastQCFastQC Report
Sat 31 Dec 2022
SRR1954716.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954716.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888855
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135191.5209454860466554No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTC35470.39905271388471686No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCG34200.38476466915301144No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC31430.35360098103740206No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.265172609705745No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGCT21130.2377215631345945No Hit
GCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC20490.2305212886241288No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCC19400.2182583210984919No Hit
CCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC14200.15975609070095798No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACGAAGT10100.113629332118287No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCT9140.10282892035258845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9120.10260391177413639No Hit
GAACTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCT9040.10170387746032818No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)