FastQCFastQC Report
Sat 31 Dec 2022
SRR1954714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251879
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28471.1303046303979292No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCG16630.6602376537940837No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTC16030.6364166921418618No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGC11290.4482310950893087No Hit
GCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC7420.2945858924324775TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC7270.288630652019422TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGCT6650.2640156583121261Illumina PCR Primer Index 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.19215575732792334No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4750.18858261308009006No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCT4340.17230495595107173No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTC4190.16634971553801625No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGCGGTTT3880.1540422186843683No Hit
GAACTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCT3750.14888101032638687No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCC3680.14610189813362764No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTA3490.13855859361042405No Hit
TCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC3250.1290302089495353TruSeq Adapter, Index 21 (95% over 23bp)
CCAGAAGGGCTACTATTACCTTTTTCTTACCATTTATACTTACCTAATGGA2830.11235553579297995No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCG2750.1091794075726837No Hit
ACTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC2530.10044505496686902No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)