FastQCFastQC Report
Sat 31 Dec 2022
SRR1954713.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954713.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101678
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.781880052715435No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCG3830.3766793209937253No Hit
GAATCTATCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTC3320.3265209779893389No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGC2790.2743956411416432No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.22325380121560218No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.2124353350774012No Hit
GCTGTCACTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC1590.156376010543087TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCACTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGC1500.14752453824819528TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTATTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTCTGCT1450.1426070536399221Illumina PCR Primer Index 1 (95% over 21bp)
GAATGAACTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTC1290.12687110289344794No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCG1110.10916815830366451No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGCGGTTTCGTATGCCGTCTTC1060.10425067369539134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)