FastQCFastQC Report
Sat 31 Dec 2022
SRR1954712.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954712.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111875
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16141.44268156424581No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG10120.9045810055865923No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC8730.7803351955307262No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC7530.673072625698324No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC7030.6283798882681564Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT5680.5077094972067039Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC4290.383463687150838Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCC3280.2931843575418995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.28782122905027935No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTGAACT2960.2645810055865922No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT2840.25385474860335194No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.24670391061452512No Hit
TCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC2670.238659217877095Illumina PCR Primer Index 5 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCT2490.22256983240223463Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC2330.20826815642458102No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTA2210.1975418994413408No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG2020.1805586592178771No Hit
ACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC1860.16625698324022345Illumina PCR Primer Index 5 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC1320.11798882681564246No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC1300.11620111731843576No Hit
GAATATGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC1240.11083798882681563No Hit
GCTGACTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC1230.10994413407821228Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)