FastQCFastQC Report
Sat 31 Dec 2022
SRR1954711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43363
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4371.007771602518276No Hit
GAATCTATCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC2390.5511611281507276No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG2030.46814104190208244No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC1740.40126375020178495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.35744759357055556No Hit
GCTGTCACTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC1340.3090192099255125Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.2882641883633512No Hit
CTGTCTATTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT1130.26059082628046953Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCACTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC870.20063187510089248Illumina PCR Primer Index 5 (95% over 23bp)
GAATGAATGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCT780.17987685353873117No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG700.1614279454834767No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTC570.1314484698936882No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC560.12914235638678137No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.11530567534534049No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC470.10838733482462007Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)