FastQCFastQC Report
Sat 31 Dec 2022
SRR1954702.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954702.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262313
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG35191.341527106929508No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC31181.188656299916512No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29921.1406220812540744No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC25380.9675464044862435No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC13150.501309504294488TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGTCTTT9490.36178153579883576No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT9240.3522509368578759No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT8070.3076477338141838No Hit
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT7760.2958297911273936TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC7220.2752436974149204No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTA7020.26761921826215246No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG6380.24322088497329528No Hit
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC5750.21920377564207646TruSeq Adapter, Index 16 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC4990.19023075486155852No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.18794341111572815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.18146260383587548No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC4360.1662136455303397No Hit
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC4200.1601140622081254TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCC4060.1547769268011879No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT4020.1532520309706343No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGT3090.11779820291026369No Hit
ACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC3040.11589208312207172TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)