FastQCFastQC Report
Sat 31 Dec 2022
SRR1954701.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954701.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100798
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGAAACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG7590.7529911307764042No Hit
GAATCTATCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC7440.7381098831326018No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.7152919700787714No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC5620.5575507450544654No Hit
GCTGTCACTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC2670.26488620805968377TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG2320.2301632968908113No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC2150.21329788289450188No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.2103216333657414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.2043691343082204No Hit
GAATGAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC2030.2013928847794599No Hit
GAATGAATGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT2010.19940871842695293No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC1720.1706383063156015No Hit
CTGTCTATTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT1610.15972539137681302TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGACTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT1580.15674914184805255No Hit
CCTGTCACTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC1330.1319470624417151TruSeq Adapter, Index 16 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1030.1021845671541102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)