FastQCFastQC Report
Sat 31 Dec 2022
SRR1954700.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954700.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613557
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59590.9712219076630206No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCG19210.3130923451284885No Hit
GAATCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTC17970.2928823238916678No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGC13240.2157908719157307No Hit
GCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC13020.2122052229866174No Hit
CTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGCT11040.1799343826245972Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9620.15679064862759287No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCC9250.15076023906499314No Hit
CCTGTCTCTTATACACATCTGACGCACATCCGTTCGTATGCCGTCTTCTGC8770.14293700503783674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6300.10267994660642776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)