FastQCFastQC Report
Sat 31 Dec 2022
SRR1954696.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954696.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388856
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.9779969963174028No Hit
CTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGCT10130.26050774579793035Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8680.22321887793939144No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC8420.21653259818544654Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC8060.20727467237229205Illumina Single End Adapter 2 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.17718641347953998No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCC6010.1545559281584957No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGC4970.1278108091427161No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC4810.12369617544798074Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCG4720.12138169399469212No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTC4600.11829571872364061No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4070.10466599460982985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)