FastQCFastQC Report
Sat 31 Dec 2022
SRR1954695.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954695.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157829
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.6526050345627229No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.2806835245740643No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.19261352476414348No Hit
CTGTCTATTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGCT1950.12355143858226308Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCACTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC1900.12038345297758968Illumina Single End Adapter 2 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1810.11468107888917753No Hit
CCTGTCACTTATACACATCTGACGCGCTTCAGTTCGTATGCCGTCTTCTGC1670.10581071919609197Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)