FastQCFastQC Report
Sat 31 Dec 2022
SRR1954692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446744
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC44430.9945293053739949RNA PCR Primer, Index 10 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG38800.8685063481546478No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC31900.7140554769621976No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30580.6845083537775549No Hit
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC29250.6547373887506044RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT25710.5754973765736081TruSeq Adapter, Index 10 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC19250.43089554644270545RNA PCR Primer, Index 10 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC11010.24644986838099672No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT10920.2444352918002256TruSeq Adapter, Index 10 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT10920.2444352918002256No Hit
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC10480.2345862507386781RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT9290.20794907150403807No Hit
ACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC8770.19630929570402736TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTA7440.1665383306770768No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG7130.15959923356553193No Hit
GAATATGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC6810.15243629461167918RNA PCR Primer, Index 10 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG5610.1255752735347313TruSeq Adapter, Index 10 (95% over 23bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5140.11505470694626004No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC5110.11438318141933634No Hit
ACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTG5100.11415933957702847TruSeq Adapter, Index 10 (95% over 23bp)
GCTGACTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC4870.10901097720394679RNA PCR Primer, Index 10 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCC4760.10654871693855988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)