FastQCFastQC Report
Sat 31 Dec 2022
SRR1954691.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954691.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167803
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC10750.6406321698658546TruSeq Adapter, Index 10 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG9010.5369391488829163No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.47376983724963206No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC7350.4380136231175843No Hit
GCTGTCACTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC5610.334320602134646RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTATTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT5550.3307449807214412TruSeq Adapter, Index 10 (96% over 25bp)
CCTGTCACTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC4350.2592325524573458RNA PCR Primer, Index 10 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC3250.19367949321525837RNA PCR Primer, Index 10 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG2860.17043795402942738No Hit
GAATGAATGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCT2740.16328671120301783No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTC2690.16030702669201383No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.14064110891938764No Hit
GAACTGACTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT2220.1322979922885765TruSeq Adapter, Index 10 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC2110.12574268636436775RNA PCR Primer, Index 35 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC2100.12514674946216695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.12514674946216695No Hit
TCTGTCACTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC2040.12157112804896217RNA PCR Primer, Index 35 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1850.11024832690714706No Hit
ACTGTCACTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC1690.10071333647193437TruSeq Adapter, Index 10 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)