FastQCFastQC Report
Sat 31 Dec 2022
SRR1954689.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954689.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156504
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.8306496958544191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5820.37187547922097836No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGC5090.3252313039922302No Hit
GAATCTATCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTC4960.31692480703368603No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCG4370.2792260900679855No Hit
CTGTCTATTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGCT4240.2709195931094413No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.2517507539743393No Hit
CCTGTCACTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC2840.18146501047896538No Hit
GCTGTCACTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC2740.17507539743393138No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2280.14568317742677503No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.11245718959259828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)