FastQCFastQC Report
Sat 31 Dec 2022
SRR1954688.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954688.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344814
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31470.9126659590387861No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG26770.7763605886071911No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC25400.7366290231835134No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC18110.5252106933013161No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC13440.3897753571490717TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT12300.35671405453374866TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC9620.2789909922450945TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT7370.21373842129379897No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT6730.19517769000098603No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT6690.19401764429518523No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6560.1902474957513326No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC6430.18647734720747997No Hit
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC5590.162116387385663TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCC5460.15834623884181037No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.15515611315085814No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA5200.1508059417541051No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG4610.1336952675935432No Hit
GAATATGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC3760.10904429634527602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)