FastQCFastQC Report
Sat 31 Dec 2022
SRR1954687.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954687.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134154
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.614219479106102No Hit
GAATCTATCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC6500.48451779298418235No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG5420.40401329814988746No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC4290.3197817433695604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3270.24374972047050406No Hit
GCTGTCACTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC2710.20200664907494373TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTATTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT2500.1863529973016086TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.17219016950668636No Hit
CCTGTCACTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC1990.14833698585208044TruSeq Adapter, Index 16 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC1820.13566498203557106No Hit
GAATGAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC1720.12821086214350672No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG1620.12075674225144237No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1530.11404803434858447No Hit
GAATGAATGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT1490.11106638639175873No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)