FastQCFastQC Report
Sat 31 Dec 2022
SRR1954686.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954686.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304587
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30631.00562400890386No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG24120.7918919717519133No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC22780.7478979733212514No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC21900.7190063922623092No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT16790.5512382340677704Illumina PCR Primer Index 8 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13990.45931047615295467No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC13430.4409249245699915RNA PCR Primer, Index 40 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC13180.432717089041883No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC12120.3979158664027027Illumina PCR Primer Index 8 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT7380.2422953047897645RNA PCR Primer, Index 40 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT7280.2390121705785211No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGAACCT7090.2327742155771586No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC6440.21143384320407632RNA PCR Primer, Index 40 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTA5780.18976515740986977No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC5350.17564768030152303No Hit
ACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC5300.17400611319590134RNA PCR Primer, Index 40 (96% over 27bp)
AAAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC5180.17006635214240923No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.1690814118790362No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.16317177029879806No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG4020.13198199529198554No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG3860.12672898055399606No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3840.12607235371174738No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC3600.11819283160476317No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC3590.11786451818363883No Hit
TCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC3340.10965668265553027RNA PCR Primer, Index 40 (96% over 27bp)
GAATATGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC3280.10768680212878423No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT3220.10571692160203816RNA PCR Primer, Index 40 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)