FastQCFastQC Report
Sat 31 Dec 2022
SRR1954685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117096
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8360.7139441142310582No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6560.5602240896358543No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC5720.48848807815809253No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG4730.4039420646307303No Hit
GAATCTATCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC4550.38857006217120993RNA PCR Primer, Index 40 (95% over 24bp)
CTGTCTATTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT2910.24851403976224634RNA PCR Primer, Index 40 (96% over 28bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.22374803579968575No Hit
CCTGTCACTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC2420.2066680330668853Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.18702602992416478No Hit
GCTGTCACTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC2180.18617202978752476RNA PCR Primer, Index 40 (96% over 27bp)
GAATGAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC1570.13407802145248343RNA PCR Primer, Index 40 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC1520.12980802076928333No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG1510.1289540206326433No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1400.11956001912960307No Hit
CGTTTTATTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC1320.11272801803648289No Hit
GAATGAATGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT1300.11102001776320285No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC1270.10845801735328278No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)