FastQCFastQC Report
Sat 31 Dec 2022
SRR1954684.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954684.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496977
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197751.3209955797584063No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC113600.7588626946172186No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG71040.4745563893099226No Hit
GAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT45730.3054823153595546RNA PCR Primer, Index 23 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT42580.28443990789437645No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT41270.275688938440604No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC32870.21957585186679554No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC31060.20748481773601063RNA PCR Primer, Index 30 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT30470.203543541417136RNA PCR Primer, Index 30 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC30430.20327633624297503No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC29460.19679661076957095RNA PCR Primer, Index 23 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTC28100.1877116348480972No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTC25960.1734161580304841No Hit
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC25610.17107811275657542RNA PCR Primer, Index 23 (96% over 25bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGT22710.15170573762990347No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT22180.14816526907227032No Hit
CGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTG21710.14502560827587865RNA PCR Primer, Index 30 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT21530.14382318499215418RNA PCR Primer, Index 30 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT17570.11736987275021593No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG17410.11630105205357197No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC14970.10000153642975142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)