FastQCFastQC Report
Sat 31 Dec 2022
SRR1954683.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954683.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574771
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57561.0014423135474824No Hit
GAATCTATCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC29110.5064625737902574RNA PCR Primer, Index 23 (95% over 22bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG17370.3022073138693497No Hit
GAACTGACTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCT10670.1856391502006886RNA PCR Primer, Index 30 (95% over 21bp)
CGTTTTATGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCT9250.16093365879628582No Hit
CGTTTCAGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTT8750.1522345421045947No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC8230.14318746074523594No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTC7070.1230055100205125No Hit
CTGTCTATTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT6580.11448037566265522RNA PCR Primer, Index 23 (96% over 26bp)
CCTGTCACTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC6430.11187064065514787RNA PCR Primer, Index 30 (95% over 23bp)
GCTGTCACTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC6330.11013081731680964RNA PCR Primer, Index 23 (96% over 25bp)
CGTTCTATCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC6240.10856497631230526No Hit
CGTTTTACTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTC5750.10003984195444794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)