FastQCFastQC Report
Sat 31 Dec 2022
SRR1954678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227642
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.9497368675376249No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC13550.5952328656399083No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG11530.506497043603553No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC8090.35538257439312604No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT6350.2789467672925032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6070.2666467523567707No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC5690.24995387494399102No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC4700.2064645364212228No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT4030.17703235782500593No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCC3800.16692877412779716No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.15990019416452148No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCT3220.14145017176092287No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTC3210.1410108855132181No Hit
TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC3000.1317858743114188No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTATAGT2780.12212157686191476No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTT2650.11641085564175328No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2560.11245727941241071No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCC2490.10938227567847761No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTA2460.10806441693536342No Hit
ACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC2380.10455012695372559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)