FastQCFastQC Report
Sat 31 Dec 2022
SRR1954676.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954676.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295052
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37501.2709624066266285No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG16140.547022219812101No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC14750.49991187993980724No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC14130.47889863481691364No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.21352168431327362No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC6130.20775998806989954Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGCAGAT5420.18369643317110204No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT4360.14777056247712267Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC4210.14268671285061618Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.13624716999037456No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT3650.12370700757832517No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTC3610.12235131434459011No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG3410.11557284817591475No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC3400.11523392486748099No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT3370.11421715494217968No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)