FastQCFastQC Report
Sat 31 Dec 2022
SRR1954674.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954674.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310365
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54201.7463309329338035No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTC25770.8303126963414044RNA PCR Primer, Index 24 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCG25290.8148470349427287No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGC18560.5980055740821291No Hit
GCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC9460.30480241006556796RNA PCR Primer, Index 43 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGCT9340.30093599471589905RNA PCR Primer, Index 24 (96% over 25bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9200.29642517680795194No Hit
CCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC7690.24777278365795113RNA PCR Primer, Index 24 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCC7330.23617353760894433No Hit
GAACTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCT7060.22747410307218918RNA PCR Primer, Index 43 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6960.22425209028079843No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCT6380.2055644160907319No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTC6030.19428737132086413No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCAGCATTT5250.16915567154801606No Hit
TCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC4820.15530101654503567RNA PCR Primer, Index 43 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAGCATTTCGTA4680.1507901986370886No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCG4380.14112416026291624No Hit
GAATATGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTC4130.1330691282844393RNA PCR Primer, Index 24 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC3280.10568201955761763RNA PCR Primer, Index 43 (96% over 25bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGC3140.10117120164967056No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)