FastQCFastQC Report
Sat 31 Dec 2022
SRR1954673.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954673.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118263
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14961.2649772118075815No Hit
GAATCTATCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTC6260.5293286995932794RNA PCR Primer, Index 43 (95% over 22bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCG5480.4633740053947557No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGC4180.35344951506388306No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.3365380550129795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3180.2688922148093656No Hit
GCTGTCACTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC2080.17587918452939635RNA PCR Primer, Index 43 (96% over 25bp)
CTGTCTATTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGCT1990.16826902750648975RNA PCR Primer, Index 43 (96% over 26bp)
GAATCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTC1740.1471297024428604RNA PCR Primer, Index 24 (95% over 21bp)
GAATGAATGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCT1490.12599037737923102No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTC1490.12599037737923102No Hit
CCTGTCACTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCTGC1450.12260808536905034RNA PCR Primer, Index 24 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGCCG1450.12260808536905034No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.11838022035632446No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGCATTTCGTATGC1280.10823334432578237No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.10738777132323718No Hit
GAACTGACTCTTATACACATCTGACGCCCAGCATTTCGTATGCCGTCTTCT1250.10569662531814686RNA PCR Primer, Index 24 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)