FastQCFastQC Report
Sat 31 Dec 2022
SRR1954670.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954670.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241938
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27531.137894832560408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7070.2922236275409402No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG6610.27321049194421715No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC6590.27238383387479437No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC5700.23559754978548222TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT5410.22361100777885243Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC5300.21906438839702733No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC4710.19467797534905637TruSeq Adapter, Index 21 (95% over 23bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4390.18145144623829246No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCC3780.15623837512089875No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2900.11986542006629797No Hit
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC2780.11490547164976152TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)