FastQCFastQC Report
Sat 31 Dec 2022
SRR1954669.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954669.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95123
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.7674274360564742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.279637942453455No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.2155104443720236No Hit
GAATCTATCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC1380.14507532352848418No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG1360.1429727826077815No Hit
CTGTCTATTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT1230.12930626662321415Illumina Single End Adapter 2 (95% over 21bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC1130.11879356201970082No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1080.11353720971794413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)