FastQCFastQC Report
Sat 31 Dec 2022
SRR1954668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185835
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21711.168240643581672No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCG9310.5009820539726101No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGC7170.3858261360884656No Hit
GAATCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTC6690.3599967713294051No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.24107407108456425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3420.18403422390830576No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCC2860.15389996502273523No Hit
CCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGC2860.15389996502273523No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAACGCGAT2550.13721850028250868No Hit
CTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGCT2200.1183845884790271Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTCTGC1940.10439368256786935No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCT1910.10277934727042806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)