FastQCFastQC Report
Sat 31 Dec 2022
SRR1954666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299741
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34771.160001467933983No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC17190.5734951174513997No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG16790.5601502630604421No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC16620.5544786999442852No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC7940.2648953596605069No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT7160.2388728935981397Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.20851334985871137No Hit
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC5520.18415899059521387No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5290.17648569932041328No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAATCGGT4950.1651425730880994No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT4460.14879512645917642No Hit
GAACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT4300.1434571847027934No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC4240.1414554565441498No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTA3500.11676747592087836No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTC3400.11343126232313898No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3380.11276401960359111No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)