FastQCFastQC Report
Sat 31 Dec 2022
SRR1954665.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954665.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121982
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9460.7755242576773622No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC3420.2803692348051352No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG3390.27790985555245856No Hit
GAATCTATCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC3200.2623337869521733No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.22872227049892607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.22052433965667065No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1510.12378875571805675No Hit
CTGTCTATTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT1380.11313144562312473Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC1290.10575330786509485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)