FastQCFastQC Report
Sat 31 Dec 2022
SRR1954664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359499
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49061.3646769532043204No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCG28800.8011148848814601No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC28100.7816433425405911No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTC27900.7760800447289145No Hit
GCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC10000.2781648905838403No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCCGAT9790.27232342788157965No Hit
CTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGCT8910.24784491751020168Illumina Single End Adapter 2 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.22308824224823992No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCT7030.19554991808043973No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCT6840.19026478515934675No Hit
CCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC6010.167177099240888No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCCGATCGTA5980.1663426045691365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.165508109897385No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCC5910.1643954503350496No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTC5890.16383912055388195No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCG5460.1518780302587768No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC4730.13157199324615645No Hit
GAATATGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTC3870.10764981265594618No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)