FastQCFastQC Report
Sat 31 Dec 2022
SRR1954663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137826
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.9932813837737438No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC6350.46072584272923833No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCG5900.42807597985866236No Hit
GAATCTATCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTC5760.41791824474337214No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.29675097586812355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.21403798992933118No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTC2030.14728715917170926No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGC1910.138580529072889No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCG1650.11971616385877847No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1600.11608840131760334No Hit
GCTGTCACTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC1580.11463729630113331No Hit
CTGTCTATTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGCT1450.10520511369407803Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph