FastQCFastQC Report
Sat 31 Dec 2022
SRR1954662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642868
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC56690.8818295513231332No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG37930.590012257570761No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31430.488902854085131No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC29100.4526590217587436TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC29020.4514145983312282No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT27200.42310396535525174Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC20650.32121679722742463TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT15060.23426271022978276No Hit
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC12740.19817443083183484TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT11200.17421927985216248No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC11090.17250819763932876No Hit
ACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC9850.15321963451283932No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT9060.1409309531661243No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT8970.1395309768101694No Hit
GAATATGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC8620.13408662431478935No Hit
GTTGAAGGGATCCATAACTCTTGATTCCAGGATTCTGATACATTCTTCTGA8140.12662008374969666No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC8030.12490900153686293No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA7730.12024241368368001No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG6880.10702041476632838No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)