FastQCFastQC Report
Sat 31 Dec 2022
SRR1954661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238144
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC11330.4757625638269283No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.3724637194302607No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG6950.2918402311206665No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC5830.24480986294006987No Hit
CTGTCTATTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT4920.20659768879333512Illumina Paired End PCR Primer 2 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC4560.1914807847352862TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCACTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC3560.14948938457403924TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC3530.14822964256920182No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.11757592045149154No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2770.11631617844665412No Hit
GTTGAAAGGATCCATAACTCTTGATTCCAGGATTCTGATACATTCTTCTGA2630.11043738242407955No Hit
GAACTGACTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT2570.10791789841440473No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC2440.10245901639344263No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG2400.10077936038699274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)