FastQCFastQC Report
Sat 31 Dec 2022
SRR1954660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849001
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96341.134745424328122No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC33560.3952881092012848No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG25480.3001174321349445No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT22580.26595963962351044Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC21660.2551233744129866No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC18310.21566523478770933No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.20129540483462327No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC16510.1944638463323365No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCC11190.13180196489756785No Hit
GAACTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCT9650.1136629992190822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)