FastQCFastQC Report
Sat 31 Dec 2022
SRR1954659.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954659.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356397
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.7264370912213065No Hit
GAATCTATCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC8570.24046218121925833No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG5990.1680709994753042No Hit
CTGTCTATTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT4910.13776771409411415Illumina Single End Adapter 2 (95% over 21bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC4880.13692595616685888No Hit
GCTGTCACTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC4580.12850837689430608No Hit
CCTGTCACTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC3710.10409739700390296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)