FastQCFastQC Report
Sat 31 Dec 2022
SRR1954656.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954656.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602411
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133892.222568976994112No Hit
GAATCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTC26570.4410610032021328No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.3814671378842684No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCG20760.34461522116960014No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGC14230.23621746614852648No Hit
GCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC11510.1910655681918159TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGCT10090.1674936214644155TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCC10070.1671616222147338No Hit
GAACTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCT8270.13728168974338117No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7870.13064170474974726No Hit
CCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTTCTGC7530.12499771750515844TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAATGCTGTCGTATGCCGTCTT6190.10275376777648482No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)