FastQCFastQC Report
Sat 31 Dec 2022
SRR1954654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405357
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.634995818500729No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG22010.5429781649262255No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC21140.5215156022962475No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC14440.3562292004331984No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC12170.3002291806975086TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT10110.24940977952767562Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC7090.17490755062821167TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT6420.15837891044190677No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC5630.138889916789398No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT5530.13642295556756143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.13148903312388832No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATACGCT5290.1305022486351537No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTC5290.1305022486351537No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTA4510.11125995110482859No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.10657272478333912No Hit
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC4310.10632602866115547TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)