FastQCFastQC Report
Sat 31 Dec 2022
SRR1954653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161746
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7100.4389598506300001No Hit
GAATCTATCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC4690.28996080274009867No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG4290.2652306703102395No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC3070.18980376639916907No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.14034350153945074No Hit
GCTGTCACTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC2200.13601572836422537TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTATTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT2090.1292149419460141Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.12859668863526766No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1760.10881258269138032No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)