FastQCFastQC Report
Sat 31 Dec 2022
SRR1954652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593813
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90501.5240488167150263No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC16570.2790440761653921No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT16440.27685483477121586Illumina PCR Primer Index 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC15710.2645614023269952TruSeq Adapter, Index 13 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15430.2598461131703078No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG13830.23290160370352284No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC13550.22818631454683547No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC11910.20056819234338083TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10130.17059242556158252No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC9520.16031983132737074No Hit
TCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC7160.12057667986386286TruSeq Adapter, Index 13 (95% over 22bp)
CTACACAAAAATAAATAAAGGTAAAAACTCTCATACGCGCATGTCATCGGG6730.1133353429446644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)