FastQCFastQC Report
Sat 31 Dec 2022
SRR1954651.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954651.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224912
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24371.0835348936472933No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.3125666927509426No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.20496905456356262No Hit
GAATCTATCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC2810.12493775343245359No Hit
CTGTCTATTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT2610.11604538664010813Illumina PCR Primer Index 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.10492992814967632No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)