FastQCFastQC Report
Sat 31 Dec 2022
SRR1954650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150416
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25911.7225561110520156No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG12990.8636049356451441No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC11830.7864854802680565No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC9770.649531964684608No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC5830.3875917455589831No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.3051537070524412No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT4280.2845441974258058No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC3470.23069354324008082No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC3420.22736942878417188No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC3340.22205084565471758No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT3260.2167322625252633No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT3260.2167322625252633No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT3040.2021061589192639No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA2850.18947452398680992No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2640.17551324327199233No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG2370.15756302521008403No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC1850.12299223486863099No Hit
GAATATGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC1750.1163440059568131No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC1700.11301989150090416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)