FastQCFastQC Report
Sat 31 Dec 2022
SRR1954646.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954646.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366289
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC52151.4237391786267128No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG50501.3786927808369895No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39551.0797485045960975No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC36240.9893827005451981No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC14830.40487156316460504TruSeq Adapter, Index 22 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT14790.40377952927879346No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC14020.3827578769769226No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT13820.37729770754786524No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAGTTAT11080.302493386369779No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT10600.2893889797400413Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA10010.27328147992432206No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG9080.24789169207920522No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC7520.20530237053255762TruSeq Adapter, Index 22 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC7190.19629309097461295No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.1799125826874408No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT6140.16762720147206167No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC5550.1515197016563424No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC4940.13486618489771737No Hit
TGTAAGGGGTAAAATTCAATTCCAAGCAACTACGAATGAAAGCTGTATGAA4630.1264029222826784No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGT4110.11220648176712923No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC4110.11220648176712923TruSeq Adapter, Index 22 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.1097494055240534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)