FastQCFastQC Report
Sat 31 Dec 2022
SRR1954645.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954645.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140464
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC13200.9397425674906026No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG11880.8457683107415422No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.7795591753047044No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC9320.6635152067433648No Hit
GAACTGACTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT3690.26270076318487295No Hit
GCTGTCACTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC3580.2548695751224513TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG3450.24561453468504385No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC3400.24205490374757943No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC3360.23920719899760795No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.22425674906025742No Hit
GAATGAATGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT2850.202898963435471No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC2830.20147511106048524No Hit
CTGTCTATTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT2430.17299806356077002Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCACTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC1800.12814671374871853TruSeq Adapter, Index 22 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1720.12245130424877548No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1410.10038159243649619No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)