FastQCFastQC Report
Sat 31 Dec 2022
SRR1954642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811195
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109631.3514629651316885No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC71310.8790734656895074TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG38720.4773204963048342No Hit
GAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT28250.3482516534248855No Hit
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG21230.26171265848532105No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGC20920.25789113591676477No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT19940.2458101936032643No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18360.226332755995784No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC17420.21474491336854884TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC17170.2116630403293906TruSeq Adapter, Index 23 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC17000.20956736666276296No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT16030.19760969927082886No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC15860.1955140256042012No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT15670.19317180209444093No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT13740.169379742232139No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT11680.1439851083894748Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC11190.13794463723272454No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC11090.13671188801706124No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCTCTGCT9260.11415257737042266No Hit
GAATATGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC8940.11020777988030005TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGT8400.10355093411571817No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT8170.10071561091969256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)